how to sort ctcs

Evaluating CTC isolation device performance

Keywords CTC: Circulating tumor cell. Read about what they are and why they’re important here. Leukocyte: White blood cell. Erythrocyte: Red blood cell. I’ve previously discussed how to sort CTCs, and the standards used to characterize device performance. Today, I’ll explain what some of the most common evaluation metrics are, and place them in context of eventual clinical/industrial application. What are common performance criteria? Capture Efficiency is number of target cells captured divided by total number of target cells introduced into the cell isolation system.

Cancer Cell Lines & CTCs: Benchmarking versus Application

x-posted at Kirby Lab Student Blog Keywords Cell Surface Marker: Some protein or receptor sticking out of the cell membrane. I explain this more in depth in my immunocapture post. CTC: Circulating tumor cell. Read about what they are and why they’re important here. Microemboli: A small mass of cells or tissue inside the bloodstream. Platelets: aka thrombocytes, important in the formation of blood clots. Phenotype: Observable characteristics of a cell.

How to Sort Circulating Tumor Cells Part IV: Electrokinetic Separation

x-posted at Kirby Lab Student Blog Keywords Anion:a negatively charged ion. Basophil, Lymphocyte, Neutrophil: Different types of white blood cells. Biofouling: Fouling of surface with biological material. Cation: A positively charged ion. Conductivity: Measure of a substance’s ability to conduct electricity. Cytoplasm: Inner contents of the cell, which holds everything outside the nucleus. CTC: Circulating tumor cell. Read about what they are and why they’re important here. Erythrocyte: Red blood cell.

How to Sort Circulating Tumor Cells Part III: Microscopic Characterization

x-posted at Kirby Lab Student Blog Keywords CTC: Circulating tumor cell. Read about what they are and why they’re important here. Cell Fixation: Chemical preservation of a cell. Post-fixation, a cell is no longer alive. Cell Staining: Using different markers to visualize cells, or components of cells. Fluorophore: A fluorescent molecule that is excited at one wavelength of light (excitation), and emits light at another wavelength (emission). Leukocyte: White blood cell (WBC).

Roundup: Circulating Tumor Cell Tech Review Papers

Keywords Biomarker: A detectable cell characteristic that tells us something about its biological state. CTC: Circulating tumor cell. Read about what they are and why they’re important here. I have been out of town most of this week at the World CTC Conference in Boston, which was a great forum to hear about the latest in CTC research, and I had a very engaged audience at my presentation. However, I didn’t have time to write up my next post in my series on sorting techniques.

How to Sort Circulating Tumor Cells Part II: Immunocapture

x-posted at Kirby Lab Student Blog Keywords Antibody: A Y-shaped protein that binds to unique cellular targets (i.e. antigens). Read more about antibody function here. Aptamer: A peptide, RNA or DNA that binds to unique cellular targets and is analogous to antibodies. Aptamers are easier to produce synthetically and are generally more stable than antibodies binding to the same target. Read more about antibodies vs. aptamers here. CTC:Circulating tumor cell.

How to Sort Circulating Tumor Cells Part I: Size

x-posted at Kirby Lab Student Blog Keywords CTC: Circulating tumor cell. Read about what they are and why they’re important here. Leukocyte: White blood cell. Erythrocyte: Red blood cell. Thrombocyte: Platelets, important in the formation of blood clots. Reynolds number: is the ratio of inertial to viscous fluid forces. In pressure-driven flow, this is the ratio of the pressure force applied (to actuate flow) as compared to the resistance of the fluid to deformation or shearing.